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Preparation of Defined Albumin–Polymer Hybrids for Efficient Cell Transfection
Author(s) -
Zöphel Lukas,
Eisele Klaus,
Gropeanu Radu,
Rouhanipour Ali,
Koynov Kaloian,
Lieberwirth Ingo,
Müllen Klaus,
Weil Tanja
Publication year - 2010
Publication title -
macromolecular chemistry and physics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.57
H-Index - 112
eISSN - 1521-3935
pISSN - 1022-1352
DOI - 10.1002/macp.200900535
Subject(s) - polyelectrolyte , transfection , chemistry , bovine serum albumin , polymer chemistry , polymer , methacrylate , linker , dimer , ethylene oxide , dna , bifunctional , biophysics , polymerization , biochemistry , copolymer , gene , organic chemistry , biology , computer science , operating system , catalysis
The preparation of two structurally defined protein–polymer hybrid architectures was presented by connecting two BSA proteins via a bifunctional poly(ethylene oxide) linker. The dimer consisting of native BSA with a negative net charge was characterized in solution as well as on surfaces, and no aggregation was observed. On a surface, defined architectures were found since both BSA subunits were efficiently separated by the PEO chain. After reaction of accessible, negatively charged amino acid residues with ethylenediamine, cBSA‐PEO dimers were achieved with a positive net charge in each subunit. These positively charged polyelectrolytes underwent complex formation with plasmid DNA coding for the luminescent enzyme luciferase. Efficient in vitro gene transfection was obtained with these protein polyelectrolytes and compared with polymer‐based gene transfection agents.