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Investigation of Nanoparticle Coating by Fluorescence Correlation Spectroscopy
Author(s) -
Jurkiewicz Piotr,
Koňák Čestmír,
Šubr Vladimír,
Hof Martin,
Štĕpánek Petr,
Ulbrich Karel
Publication year - 2008
Publication title -
macromolecular chemistry and physics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.57
H-Index - 112
eISSN - 1521-3935
pISSN - 1022-1352
DOI - 10.1002/macp.200800023
Subject(s) - methacrylamide , coating , copolymer , polymer , fluorescence , nanoparticle , polymer chemistry , materials science , chemistry , chemical engineering , nanotechnology , organic chemistry , optics , acrylamide , physics , engineering
Protecting gene delivery vectors by hydrophilic copolymer coating is a promising approach in targeted gene therapy. Herein we demonstrate the usefulness of FCS in characterizing the coating process of a virus model – latex beads with coumarin‐labeled N‐ (2‐hydroxypropyl)methacrylamide copolymer containing thiazolidine‐2‐thione groups. FCS gives an estimation of the average number of polymer chains coating the latex bead, which agrees with light scattering results, giving an evidence of quality of the vector surface modification. FCS is advantageous because it decreases the sample consumption by a factor of 1 000. The coumarin label showed advantages over the previously used fluorescein‐based one.