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Chromatic Transitions in Polydiacetylene Langmuir‐Blodgett Films due to Molecular Recognition at the Film Surface Studied by Spectroscopic Methods and Surface Analysis
Author(s) -
Geiger Elisabeth,
Hug Paul,
Keller Beat A.
Publication year - 2003
Publication title -
macromolecular chemistry and physics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.57
H-Index - 112
eISSN - 1521-3935
pISSN - 1022-1352
DOI - 10.1002/macp.200290025
Subject(s) - diacetylene , chemistry , streptavidin , hydroxymethyl , polydiacetylenes , langmuir–blodgett film , analytical chemistry (journal) , bromophenol blue , x ray photoelectron spectroscopy , raman spectroscopy , monomer , nuclear chemistry , chromatography , organic chemistry , molecule , biotin , chemical engineering , polymer , engineering , biochemistry , physics , optics
Langmuir‐Blodgett films of polydiacetylene are used to create a biosensor on the basis of a surface host‐guest recognition process. Functionalizing a 10 wt.‐% fraction of the diacetylene monomer with biotin leads to a visually observable color change in the resulting polymerized film when streptavidin in the molar ratio streptavidin:biotin = 1:2 is added. The chromatic transition, caused by a shortening of the conjugation length of the π ‐electron system in the polydiacetylene backbone, is investigated by monitoring the change of visible optical absorption and Raman spectroscopy. Chemical species and to some extent also the presence of surface‐immobilized peptide are identified via time‐of‐flight secondary‐ion mass spectrometry (TOF‐SIMS) signal assignment and XPS studies.Optical spectra of the blue LB film consisting of two layers PDA 1 and one layer of a mixture containing 10 wt.‐% 7 in 1 , the film after 10 min immersion in tris(hydroxymethyl)aminomethane hydrochloride buffer (pH 7.3 at 21 °C) and after 2 h immersion in streptavidin solution (protein:biotin molar ratio = 1:2).