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Immobilization of enzymes by multilayer microcapsules
Author(s) -
Pommersheim Rainer,
Schrezenmeir Jürgen,
Vogt Walter
Publication year - 1994
Publication title -
macromolecular chemistry and physics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.57
H-Index - 112
eISSN - 1521-3935
pISSN - 1022-1352
DOI - 10.1002/macp.1994.021950508
Subject(s) - membrane , enzyme , chemistry , immobilized enzyme , phosphate , permeability (electromagnetism) , polymer chemistry , chemical engineering , acid phosphatase , acrylic acid , materials science , copolymer , organic chemistry , biochemistry , polymer , engineering
A method is described to obtain a new type of microcapsules for immobilization of enzymes or living cells. The wall of these capsules consists of several layers of poly(ethyleneimine) and poly(acrylic acid). The idea is that diverging properties of the whole assembly can be better controlled when the membrane is built up by several consecutive steps, each being optimized with respect to a special property, for example, permeability or mechanical strength. The encapsulation of acidic phosphatase and the cleavage of p ‐nitrophenyl phosphate was used as a model system. The charged capsules were characterized by their enzymatic activity, as a function of membrane composition (number and sequence of layers) and storing time. The permeability for the substrate and the retaining ability for the enzyme were also measured.