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Nanoparticles Composed of PEGylated Alternating Copolymer‐Combretastatin A4 Conjugate for Cancer Therapy
Author(s) -
Zhang Yu,
Liu Xinming,
Wang Xueping,
He Pan,
Xiao Chunsheng,
Yu Haiyang,
Chen Xuesi
Publication year - 2021
Publication title -
macromolecular bioscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.924
H-Index - 105
eISSN - 1616-5195
pISSN - 1616-5187
DOI - 10.1002/mabi.202100077
Subject(s) - copolymer , conjugate , chemistry , in vivo , click chemistry , cytotoxicity , nanoparticle , drug delivery , in vitro , materials science , combinatorial chemistry , nanotechnology , polymer , organic chemistry , biochemistry , mathematical analysis , mathematics , microbiology and biotechnology , biology
Chemotherapy using vascular targeting agents is an emerging new approach for cancer therapy. Combretastatin A4 (CA4) is a leading vascular‐disrupting agent that targets the tumor blood vasculature for clinical tumor elimination. However, the extremely poor water solubility of CA4 hinders its biomedical applications. In this study, nanoparticles composed of novel PEGylated alternating copolymer‐CA4 conjugates are designed to improve the therapeutic efficiency of CA4. First, an alternating copolymer with an alkene‐pendant is synthesized by mPEG‐OH‐initiated ring‐opening copolymerization. Then, side carboxyl groups are introduced by a thio‐ene “click” chemical reaction, followed with CA4 conjugation through the Yamaguchi‐reaction, resulting in the target copolymer, mPEG‐ b ‐P(PA‐ alt ‐GCA4). Interestingly, the polymer‐drug conjugates can self‐assemble into nanoparticles with an average diameter of 55.6 nm. The in vitro drug release and cytotoxicity of the obtained CA4‐NPs toward 4T1 cells are investigated. Finally, the antitumor efficiency is evaluated in a 4T1‐tumor bearing murine model. The in vivo test results demonstrate that CA4‐NPs inhibited tumor growth much more efficiently at doses of 30 and 60 mg kg −1 , compared with the control group.