Premium
Fluoropolymer‐Mediated Intracellular Delivery of miR‐23b for the Osteocyte Differentiation in Osteoblasts
Author(s) -
Wang Sihan,
Xing Jiakai,
Xiong Boyu,
Han Haobo,
Hu Min,
Li Quanshun
Publication year - 2021
Publication title -
macromolecular bioscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.924
H-Index - 105
eISSN - 1616-5195
pISSN - 1616-5187
DOI - 10.1002/mabi.202100024
Subject(s) - osteopontin , transfection , runx2 , chemistry , osteoblast , microrna , intracellular , osteocyte , microbiology and biotechnology , osteocalcin , alkaline phosphatase , cellular differentiation , gene knockdown , biochemistry , biology , gene , endocrinology , in vitro , enzyme
Emerging evidence suggests that microRNAs (miRNAs) play key roles in the regulation of multiple biological processes, including the differentiation of osteoblasts. Although miRNA‐based gene therapy holds immense potential in the treatment of a variety of diseases, the intracellular delivery of miRNA remains challenging owing to the lack of efficient and safe gene carriers. In this study, a fluoropolymer (FP) is constructed through the modification of polyamidoamine (PAMAM) using heptafluorobutyric anhydride and then is used as a carrier for miR‐23b transfection to induce osteocyte differentiation of osteoblasts. The derivative FP is found to facilitate miR‐23b transfection due to its favorable endosomal escape from the "proton sponge" effect. Compared to PAMAM/miR‐23b, the FP/miR‐23b nanocomplex efficiently promotes the differentiation of osteoblasts and formation of calcified nodules, attributable to enhanced expression of various osteogenesis genes (runt‐related transfection factor 2 [RUNX2], alkaline phosphatase [ALP], osteopontin [OPN], and osteocalcin [OCN]). Thus, FP‐mediated miR‐23b transfection may be used as an effective strategy to facilitate osteogenic differentiation.