Premium
Comparison Study of the Effects of Cationic Liposomes on Delivery across 3D Skin Tissue and Whitening Effects in Pigmented 3D Skin
Author(s) -
Lee Mi So,
Lee Ju Won,
Kim Su Ji,
PhamNguyen OanhVu,
Park Jongmin,
Park Ju Hyun,
Jung Young Mee,
Lee Jun Bae,
Yoo Hyuk Sang
Publication year - 2021
Publication title -
macromolecular bioscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.924
H-Index - 105
eISSN - 1616-5195
pISSN - 1616-5187
DOI - 10.1002/mabi.202000413
Subject(s) - liposome , permeation , chemistry , stratum corneum , biophysics , zeta potential , cationic liposome , dynamic light scattering , membrane , chromatography , materials science , nanotechnology , biochemistry , transfection , pathology , medicine , nanoparticle , gene , biology
Charged phospholipids are employed to formulate liposomes with different surface charges to enhance the permeation of active ingredients through epidermal layers. Although 3D skin tissue is widely employed as an alternative to permeation studies using animal skin, only a small number of studies have compared the difference between these skin models. Liposomal delivery strategies are investigated herein, through 3D skin tissue based on their surface charges. Cationic, anionic, and neutral liposomes are formulated and their size, zeta‐potential, and morphology are characterized using dynamic light scattering and cryogenic‐transmission electron microscopy (cryo‐TEM). A Franz diffusion cell is employed to determine the delivery efficiency of various liposomes, where all liposomes do not exhibit any recognizable difference of permeation through the synthetic membrane. When the fluorescence liposomes are applied to 3D skin, considerable fluorescence intensity is observed at the stratum cornea and epithelium layers. Compared to other liposomes, cationic liposomes exhibit the highest fluorescence intensity, suggesting the enhanced permeation of liposomes through the 3D skin layers. Finally, the ability of niacinamide (NA)‐incorporated liposomes to suppress melanin transfer in pigmented 3D skin is examined, where cationic liposomes exhibit the highest degree of whitening effects.