Premium
Biomimetic Hydrogels Loaded with Nanofibers Mediate Sustained Release of pDNA and Promote In Situ Bone Regeneration
Author(s) -
Huang Lin,
Zhang Zhijie,
Guo Mingtao,
Pan Cile,
Huang Zhiguan,
Jin Junfei,
Li Yuhe,
Hou Xiaohui,
Li Wenqiang
Publication year - 2021
Publication title -
macromolecular bioscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.924
H-Index - 105
eISSN - 1616-5195
pISSN - 1616-5187
DOI - 10.1002/mabi.202000393
Subject(s) - scaffold , regeneration (biology) , self healing hydrogels , biomedical engineering , nanofiber , gelatin , chemistry , composite number , materials science , bone healing , tissue engineering , bone morphogenetic protein 2 , biophysics , microbiology and biotechnology , nanotechnology , composite material , anatomy , in vitro , polymer chemistry , biochemistry , medicine , biology
Polymer hydrogels are generally insufficient biomechanics, strong resistance to cell adhesion, and weak bioactivity which limits their application in bone tissue engineering considerably. In order to develop a bone tissue engineering material with both good mechanical properties, osteogenic and angiogenic activity. Nanofibers carrying DNA plasmid (pNF) are introduced to gelatin methacryloyl (GelMA) and thiolated chitosan (TCS) system for preparing a novel GelMA/TCS/pNF composite hydrogel with dual network structure. By characterization of the compressive measurements, the resulting composite scaffold shows greatly enhanced mechanical strength (0.53 MPa) and is not damaged after 20 cycles of compression. And the fabricated composite scaffold displays sustained release of bone morphogenetic protein‐2 that can induce osteogenic differentiation and angiopoietin‐1 that promotes vascularization. The cell experiment shows that this system can significantly promote MC3T3‐E1 cell attachment, proliferation, as well as osteogenic‐related and angiogenic‐related genes expression of MC3T3‐E1 cells. Moreover, the in vivo results show that the composite scaffold with activated gene fibers can significantly promote osteogenesis and vascularization leading to favorable capacity of bone regeneration, meaning that the resulting biomimetic composite hydrogel scaffolds are excellent candidates for bone repair materials.