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Polymer–Protein Hybrid Network Involving Mucin: A Mineralized Biomimetic Template for Bone Tissue Engineering
Author(s) -
Barik Debyashreeta,
Bejugam Pruthvi Raj,
Nayak Chumki,
Mohanty Khirabdhi Tanaya,
Singha Achintya,
Declercq Heidi A.,
Dash Mamoni
Publication year - 2021
Publication title -
macromolecular bioscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.924
H-Index - 105
eISSN - 1616-5195
pISSN - 1616-5187
DOI - 10.1002/mabi.202000381
Subject(s) - scaffold , mucin , chemistry , regeneration (biology) , in silico , tissue engineering , self healing hydrogels , polyethylene glycol , biophysics , biochemistry , microbiology and biotechnology , biomedical engineering , biology , polymer chemistry , engineering , gene
Biomimetic matrices offer a great advantage to understand several biological processes including regeneration. The study involves the development of a hybrid biomimetic scaffold and the uniqueness lies in the use of mucin, as a constituent protein. Through this study, the role of the protein in bone regeneration is deciphered through its development as a 3D model. As a first step towards understanding the protein, the interactions of mucin and collagen are determined by in silico studies considering that collagen is the most abundant protein in the bone microenvironment. Both proteins are reported to be involved in bone biology though the exact role of mucin is a topic of investigation. The in silico studies of collagen–mucin suggest to have a proper affinity toward each other, forming a strong basis for 3D scaffold development. The developed 3D scaffold is a double network system comprising of mucin and collagen and vinyl end functionalized polyethylene glycol. In situ deposition of mineral crystals has been performed enzymatically. Biological evaluation of these mineral deposited scaffolds is done in terms of their bone regeneration potential and a comparison of the two systems with and without mineral deposition is presented.