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A Ninhydrin‐Type Urea Sorbent for the Development of a Wearable Artificial Kidney
Author(s) -
Jong Jacobus A. W.,
Guo Yong,
Hazenbrink Diënty,
Douka Stefania,
Verdijk Dennis,
Zwan Johan,
Houben Klaartje,
Baldus Marc,
Scheiner Karina C.,
Dalebout Remco,
Verhaar Marianne C.,
Smakman Robert,
Hennink Wim E.,
Gerritsen Karin G.F.,
Nostrum Cornelus F.
Publication year - 2020
Publication title -
macromolecular bioscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.924
H-Index - 105
eISSN - 1616-5195
pISSN - 1616-5187
DOI - 10.1002/mabi.201900396
Subject(s) - ninhydrin , urea , chemistry , sorbent , chromatography , nuclear chemistry , polymer chemistry , organic chemistry , biochemistry , adsorption , amino acid
The aim of this study is to develop polymeric chemisorbents with a high density of ninhydrin groups, able to covalently bind urea under physiological conditions and thus potentially suitable for use in a wearable artificial kidney. Macroporous beads are prepared by suspension polymerization of 5‐vinyl‐1‐indanone (vinylindanone) using a 90:10 (v/v) mixture of toluene and nitrobenzene as a porogen. The indanone groups are subsequently oxidized in a one‐step procedure into ninhydrin groups. Their urea absorption kinetics are evaluated under both static and dynamic conditions at 37 °C in simulated dialysate (urea in phosphate buffered saline). Under static conditions and at a 1:1 molar ratio of ninhydrin: urea the sorbent beads remove ≈0.6–0.7 mmol g −1 and under dynamic conditions and at a 2:1 molar excess of ninhydrin ≈0.6 mmol urea g −1 sorbent in 8 h at 37 °C, which is a step toward a wearable artificial kidney.