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Modulation of Living Cell Behavior with Ultra‐Low Fouling Polymer Brush Interfaces
Author(s) -
Víšová Ivana,
Smolková Barbora,
Uzhytchak Mariia,
Vrabcová Markéta,
Zhigunova Yulia,
Houska Milan,
Surman František,
los Santos Pereira Andres,
Lunov Oleg,
Dejneka Alexandr,
VaisocherováLísalová Hana
Publication year - 2020
Publication title -
macromolecular bioscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.924
H-Index - 105
eISSN - 1616-5195
pISSN - 1616-5187
DOI - 10.1002/mabi.201900351
Subject(s) - methacrylamide , fouling , wetting , polymer , copolymer , chemical engineering , biofouling , surface modification , surface charge , materials science , chemistry , nanotechnology , polymer chemistry , acrylamide , membrane , composite material , biochemistry , engineering
Ultra‐low fouling and functionalizable coatings represent emerging surface platforms for various analytical and biomedical applications such as those involving examination of cellular interactions in their native environments. Ultra‐low fouling surface platforms as advanced interfaces enabling modulation of behavior of living cells via tuning surface physicochemical properties are presented and studied. The state‐of‐art ultra‐low fouling surface‐grafted polymer brushes of zwitterionic poly(carboxybetaine acrylamide), nonionic poly( N ‐(2‐hydroxypropyl)methacrylamide), and random copolymers of carboxybetaine methacrylamide (CBMAA) and HPMAA [p(CBMAA‐ co ‐HPMAA)] with tunable molar contents of CBMAA and HPMAA are employed. Using a model Huh7 cell line, a systematic study of surface wettability, swelling, and charge effects on the cell growth, shape, and cytoskeleton distribution is performed. This study reveals that ultra‐low fouling interfaces with a high content of zwitterionic moieties (>65 mol%) modulate cell behavior in a distinctly different way compared to coatings with a high content of nonionic HPMAA. These differences are attributed mostly to the surface hydration capabilities. The results demonstrate a high potential of carboxybetaine‐rich ultra‐low fouling surfaces with high hydration capabilities and minimum background signal interferences to create next‐generation bioresponsive interfaces for advanced studies of living objects.