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Temperature‐Sensitive Amphiphilic Non‐Ionic Triblock Copolymers for Enhanced In Vivo Skeletal Muscle Transfection
Author(s) -
Rasolonjatovo Bazoly,
Illy Nicolas,
Bennevault Véronique,
Mathé Jérôme,
Midoux Patrick,
Le Gall Tony,
Haudebourg Thomas,
Montier Tristan,
Lehn Pierre,
Pitard Bruno,
Cheradame Herve,
Huin Cécile,
Guégan Philippe
Publication year - 2020
Publication title -
macromolecular bioscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.924
H-Index - 105
eISSN - 1616-5195
pISSN - 1616-5187
DOI - 10.1002/mabi.201900276
Subject(s) - amphiphile , transfection , biophysics , bilayer , skeletal muscle , in vivo , lipid bilayer , copolymer , chemistry , ionic bonding , phase transition , materials science , biochemistry , membrane , gene , polymer , organic chemistry , biology , anatomy , genetics , ion , physics , quantum mechanics
It is reported that low concentration of amphiphilic triblock copolymers of pMeOx‐ b ‐pTHF‐ b ‐pMeOx structure (TBCPs) improves gene expression in skeletal muscle upon intramuscular co‐injection with plasmid DNA. Physicochemical studies carried out to understand the involved mechanism show that a phase transition of TBCPs under their unimer state is induced when the temperature is elevated from 25 to 37 °C, the body temperature. Several lines of evidences suggest that TBCP insertion in a lipid bilayer causes enough lipid bilayer destabilization and even pore formation, a phenomenon heightened during the phase transition of TBCPs. Interestingly, this property allows DNA translocation across the lipid bilayer model. Overall, the results indicate that TBCPs exhibiting a phase transition at the body temperature is promising to favor in vivo pDNA translocation in skeletal muscle cells for gene therapy applications.