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Validation of an MPC Polymer Coating to Attenuate Surface‐Induced Crosstalk between the Complement and Coagulation Systems in Whole Blood in In Vitro and In Vivo Models
Author(s) -
Asif Sana,
Asawa Kenta,
Inoue Yuuki,
Ishihara Kazuhiko,
Lindell Björn,
Holmgren Robin,
Nilsson Bo,
Rydén Anneli,
JensenWaern Marianne,
Teramura Yuji,
Ekdahl Kristi.
Publication year - 2019
Publication title -
macromolecular bioscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.924
H-Index - 105
eISSN - 1616-5195
pISSN - 1616-5187
DOI - 10.1002/mabi.201800485
Subject(s) - complement system , in vivo , phosphorylcholine , fibrin , materials science , biomedical engineering , polymer , heparin , biomaterial , thrombogenicity , coating , chemistry , platelet , biophysics , nanotechnology , immunology , medicine , composite material , immune system , biochemistry , microbiology and biotechnology , biology
Artificial surfaces that come into contact with blood induce an immediate activation of the cascade systems of the blood, leading to a thrombotic and/or inflammatory response that can eventually cause damage to the biomaterial or the patient, or to both. Heparin coating has been used to improve hemocompatibility, and another approach is 2‐methacryloyloxyethyl phosphorylcholine (MPC)‐based polymer coatings. Here, the aim is to evaluate the hemocompatibility of MPC polymer coating by studying the interactions with coagulation and complement systems using human blood in vitro model and pig in vivo model. The stability of the coatings is investigated in vitro and MPC polymer‐coated catheters are tested in vivo by insertion into the external jugular vein of pigs to monitor the catheters' antithrombotic properties. There is no significant activation of platelets or of the coagulation and complement systems in the MPC polymer‐coated one, which was superior in hemocompatibility to non‐coated matrix surfaces. The protective effect of the MPC polymer coat does not decline after incubation in human plasma for up to 2 weeks. With MPC polymer‐coated catheters, it is possible to easily draw blood from pig for 4 days in contrast to the case for non‐coated catheters, in which substantial clotting is seen.