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Nanoparticle‐Based Imaging of Clinical Transplant Populations Encapsulated in Protective Polymer Matrices
Author(s) -
Adams Christopher F.,
Delaney Alexander M.,
Carwardine Darren R.,
Tickle Jacqueline,
Granger Nicolas,
Chari Divya M.
Publication year - 2019
Publication title -
macromolecular bioscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.924
H-Index - 105
eISSN - 1616-5195
pISSN - 1616-5187
DOI - 10.1002/mabi.201800389
Subject(s) - transplantation , viability assay , magnetic resonance imaging , tissue engineering , in vivo , cell , regeneration (biology) , biomedical engineering , medicine , chemistry , surgery , microbiology and biotechnology , biology , radiology , biochemistry
A recent clinical trial proves that autologous olfactory mucosal cell (OMC) transplantation improves locomotion in dogs with naturally occurring spinal injuries comparable to human lesions. However, not all dogs respond to the treatment, likely due to the transplantation procedures involving injections of cell suspensions that are associated with cell death, uneven cell distribution, and cell washout. Encapsulating cells in protective hydrogel matrices offers a tissue engineering solution to safely achieve 3D growth of viable transplant cells for implantation into injury sites, to improve regenerative outcomes. It is shown for the first time that canine OMCs (cOMCs) can be propagated with high viability in 3D collagen matrices. Further, a method to incorporate cOMCs pre‐labeled with clinical‐grade iron oxide nanoparticles into the constructs is described. Intraconstruct labeled cells are visualized using magnetic resonance imaging, offering substantial promise for in vivo tracking of cOMCs delivered in protective matrices.