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Synthetic, Chemically Defined Polymer‐Coated Microcarriers for the Expansion of Human Mesenchymal Stem Cells
Author(s) -
Krutty John D.,
Dias Andrew D.,
Yun Junsu,
Murphy William L.,
Gopalan Padma
Publication year - 2019
Publication title -
macromolecular bioscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.924
H-Index - 105
eISSN - 1616-5195
pISSN - 1616-5187
DOI - 10.1002/mabi.201800299
Subject(s) - microcarrier , glycidyl methacrylate , surface modification , chemistry , ethylene glycol , polymer chemistry , methacrylate , protein adsorption , mesenchymal stem cell , adhesion , polymer , methyl acrylate , chemical engineering , monomer , organic chemistry , biochemistry , cell , microbiology and biotechnology , biology , engineering
Mesenchymal stem cells (MSC), also called marrow stromal cells, are adult cells that have attracted interest for their potential uses in therapeutic applications. There is a pressing need for scalable culture systems due to the large number of cells needed for clinical treatments. Here, a tailorable thin polymer coating—poly(poly(ethylene glycol) methyl ether methacrylate‐ ran ‐vinyl dimethyl azlactone‐ ran ‐glycidyl methacrylate) [P(PEGMEMA‐ r ‐VDM‐ r ‐GMA); PVG]—to the surface of commercially available polystyrene and glass microcarriers to create chemically defined surfaces for large‐scale cell expansion is applied. These chemically defined microcarriers create a reproducible surface that does not rely on the adsorption of xenogenic serum proteins to mediate cell adhesion. Specifically, this coating method anchors PVG copolymer through ring opening nucleophilic attack by amine residues on poly‐ l ‐lysine that is pre‐adsorbed to the surface of microcarriers. Importantly, this anchoring reaction preserves the monomer VDM reactivity for subsequent functionalization with an integrin‐specific Arg‐Gly‐Asp peptide to enable cell adhesion and expansion via a one‐step reaction in aqueous media. MSCs cultured on PVG‐coated microcarriers achieve sixfold expansion—similar to the expansion achieved on PS microcarriers—and retain their ability to differentiate after harvesting.