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Hybrid Synthetic‐Biological Hydrogel System for Adipose Tissue Regeneration
Author(s) -
Li Shue,
Poche John Nicholas,
Liu Yiming,
Scherr Thomas,
McCann Jacob,
Forghani Anoosha,
Smoak Mollie,
Muir Mitchell,
Berntsen Lisa,
Chen Cong,
Ravnic Dino J.,
Gimble Jeffrey,
Hayes Daniel J.
Publication year - 2018
Publication title -
macromolecular bioscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.924
H-Index - 105
eISSN - 1616-5195
pISSN - 1616-5187
DOI - 10.1002/mabi.201800122
Subject(s) - regeneration (biology) , adipose tissue , self healing hydrogels , chemistry , tissue engineering , microbiology and biotechnology , biomedical engineering , biology , biochemistry , polymer chemistry , engineering
Hydrogels are promising scaffolds for adipose tissue regeneration. Currently, the incorporation of bioactive molecules in hydrogel system is used, which can increase the cell proliferation rate or improve adipogenic differentiation performance of stromal stem cells but often suffers from high expense or cytotoxicity because of light/thermal curing used for polymerization. In this study, decellularized adipose tissue is incorporated, at varying concentrations, with a thiol‐acrylate fraction that is then polymerized to produce hydrogels via a Michael addition reaction. The results reveal that the major component of isolated adipose‐derived extracellular matrix (ECM) is Collagen I. Mechanical properties of ECM polyethylene glycol (PEG) are not negatively affected by the incorporation of ECM. Additionally, human adipose‐derived stem cells (hASCs) are encapsulated in ECM PEG hydrogel with ECM concentrations varying from 0% to 1%. The results indicate that hASCs maintained the highest viability and proliferation rate in 1% ECM PEG hydrogel with most lipids formation when cultured in adipogenic conditions. Furthermore, more adipose regeneration is observed in 1% ECM group with in vivo study by Day 14 compared to other ECM PEG hydrogels with lower ECM content. Taken together, these findings suggest the ECM PEG hydrogel is a promising substitute for adipose tissue regeneration applications.