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Droplet‐Based Microfluidic Templating of Polyglycerol‐Based Microgels for the Encapsulation of Cells: A Comparative Study
Author(s) -
Kapourani Era,
Neumann Falko,
Achazi Katharina,
Dernedde Jens,
Haag Rainer
Publication year - 2018
Publication title -
macromolecular bioscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.924
H-Index - 105
eISSN - 1616-5195
pISSN - 1616-5187
DOI - 10.1002/mabi.201800116
Subject(s) - bioorthogonal chemistry , cell encapsulation , microfluidics , polyethylene glycol , self healing hydrogels , chemistry , nanotechnology , chemical engineering , polymer chemistry , materials science , click chemistry , organic chemistry , engineering
Cell microencapsulation holds great promise as a therapeutic strategy for the controlled and sustained delivery of biologically relevant agents. The authors developed cell‐laden microgel scaffolds with excellent long‐term viabilities by combining bioorthogonal strain promoted azide–alkyne cycloaddition (SPAAC) and droplet‐based microfluidic templating. Star‐shaped polyglycerol hexaazide, α,ω‐bis azido‐linear polyglycerol or polyethylene glycol as well as dendritic polyglycerol‐(polycyclooctyne) served as bioinert hydrogel precursors. The authors demonstrate for the first time the generation of entirely polyglycerol‐based microcapsules with excellent stability and full retention of viability of the packed cells for longer than 3 weeks. As a result, our microgel particles could be used for long‐term immunoisolation of cells enabling their study during encapsulation.