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Maintenance of Cartilaginous Gene Expression of Serially Subcultured Chondrocytes on Poly(2‐Methoxyethyl Acrylate) Analogous Polymers
Author(s) -
Hoshiba Takashi,
Maruyama Hiroka,
Sato Kazuhiro,
Endo Chiho,
Kawazoe Naoki,
Chen Guoping,
Tanaka Masaru
Publication year - 2017
Publication title -
macromolecular bioscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.924
H-Index - 105
eISSN - 1616-5195
pISSN - 1616-5187
DOI - 10.1002/mabi.201700297
Subject(s) - subculture (biology) , chondrocyte , cartilage , integrin , chemistry , acrylate , ethyl acrylate , microbiology and biotechnology , adhesion , cell adhesion , tissue engineering , polymer chemistry , cell , biochemistry , polymer , biomedical engineering , in vitro , biology , anatomy , organic chemistry , medicine , monomer
Chondrocytes are important for cartilage tissue engineering. However, dedifferentiation during chondrocyte subculture prevents the application of cartilage tissue engineering. Therefore, prevention of this dedifferentiation is required. Here, the possibility of poly(2‐methoxyethyl acrylate) (PMEA) and its analogous polymers, poly(tetrahydrofurfuryl acrylate) (PTHFA) and poly(2‐(2‐methoxyethoxy) ethyl acrylate‐ co ‐butyl acrylate) (PMe2A), for chondrocyte subculture without dedifferentiation is examined. Chondrocytes spread on PTHFA and polyethylene terephthalate (PET), whereas their spreading is delayed on PMEA and PMe2A. When primary chondrocytes are subcultured on these polymers, the expression levels of cartilaginous genes are higher on PMEA and PMe2A than on PET and PTHFA. Integrin contribution to the initial cell adhesion is lower on PMEA and PMe2A than on PTHFA and PET. This low level of integrin contribution to cell adhesion may cause a delay in cell spreading and the maintenance of cartilaginous gene expression. These results indicate that PMEA and PMe2A may be favorable substrates for chondrocyte subculture and cartilage tissue engineering.