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A Versatile Method to Prepare Protein Nanoclusters for Drug Delivery
Author(s) -
Wang Yupeng,
Yan Lesan,
He Shasha,
Zhou Dongfang,
Cheng Yanxiang,
Chen Xuesi,
Jing Xiabin,
Huang Yubin
Publication year - 2018
Publication title -
macromolecular bioscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.924
H-Index - 105
eISSN - 1616-5195
pISSN - 1616-5187
DOI - 10.1002/mabi.201700282
Subject(s) - nanoclusters , nanocarriers , isoelectric point , drug delivery , nanomedicine , nanoparticle , nanotechnology , chemistry , drug , targeted drug delivery , denaturation (fissile materials) , drug carrier , materials science , biochemistry , psychology , psychiatry , nuclear chemistry , enzyme
Abstract Nanocarriers based on natural biomaterials such as peptides and proteins have shown great advantages in the field of nanomedicine. However, the complicated preparation process and possible denaturation of proteins may limit their further applications. Herein, a novel method is developed to prepare protein nanocluster drug delivery system based on the self‐aggregated property of proteins under the isoelectric point condition. The crosslinked protein nanoclusters, prepared by adding modified natural crosslinking agent polysaccharide, exhibit excellent stability and autofluorescent property in physiological conditions. Hemoglobin, a model protein, is chosen for preparation of drug‐loaded nanoclusters. The as‐prepared nanoclusters demonstrate a pH‐responsive drug release behavior and can successfully deliver drugs into cancer cells. Moreover, this approach can be extended to various proteins, exemplifying the universal applicability of our new preparation method for protein‐based nanoparticles.