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Electroactive 3D Scaffolds Based on Silk Fibroin and Water‐Borne Polyaniline for Skeletal Muscle Tissue Engineering
Author(s) -
Zhang Mengyao,
Guo Baolin
Publication year - 2017
Publication title -
macromolecular bioscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.924
H-Index - 105
eISSN - 1616-5195
pISSN - 1616-5187
DOI - 10.1002/mabi.201700147
Subject(s) - fibroin , c2c12 , myogenesis , chemistry , tissue engineering , silk , skeletal muscle , regeneration (biology) , biocompatibility , myocyte , polyaniline , microbiology and biotechnology , biophysics , biomedical engineering , anatomy , biochemistry , materials science , biology , in vitro , organic chemistry , polymer , medicine , composite material , polymerization
Silk fibroin (SF) with good biocompatibility and degradability has great potential for tissue engineering. However, the SF based scaffolds lack the electroactivity to regulate the myogenic differentiation for the regeneration of muscle tissue, which is sensitive to electrical signal. Herein, a series of electroactive biodegradable scaffolds based on SF and water‐soluble conductive poly(aniline‐ co ‐ N ‐(4‐sulfophenyl) aniline) (PASA) via a green method for skeletal muscle tissue engineering are designed. SF/PASA scaffolds are prepared by vortex of aqueous solution of SF and PASA under physiological condition. Murine‐derived L929 fibroblast and C2C12 myoblast cells are used to evaluate cytotoxicity of SF/PASA scaffolds. Moreover, myogenic differentiation of C2C12 cells is investigated by analyzing the morphology of myotubes and related gene expression. These results suggest that electroactive SF/PASA scaffolds with a suitable microenvironment, which can enhance the myogenic differentiation of C2C12 cells, have a great potential for skeletal muscle regeneration.