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Uptake of Retinoic Acid‐Modified PMMA Nanoparticles in LX‐2 and Liver Tissue by Raman Imaging and Intravital Microscopy
Author(s) -
Yildirim Turgay,
Matthäus Christian,
Press Adrian T.,
Schubert Stephanie,
Bauer Michael,
Popp Jürgen,
Schubert Ulrich S.
Publication year - 2017
Publication title -
macromolecular bioscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.924
H-Index - 105
eISSN - 1616-5195
pISSN - 1616-5187
DOI - 10.1002/mabi.201700064
Subject(s) - nanoparticle , fluorescence microscope , chemistry , cationic polymerization , raman spectroscopy , covalent bond , microscopy , retinoic acid , biophysics , fluorescence , nanotechnology , materials science , polymer chemistry , biochemistry , organic chemistry , pathology , biology , optics , physics , gene , medicine
A primary amino‐functionalized methyl methacrylate‐based statistical copolymer is covalently coupled with retinoic acid (RA) and a fluorescent dye (DY590) in order to investigate the feasibility of the RA containing polymeric nanoparticles for Raman imaging studies and to study the possible selectivity of RA for hepatic stellate cells via intravital microscopy. Cationic nanoparticles are prepared by utilizing the nanoprecipitation method using modified polymers. Raman studies show that RA functional nanoparticles can be detectable in all tested cells without any need of additional label. Moreover, intravital microscopy indicates that DY590 is eliminated through the hepatobiliary route but not if used as covalently attached tracing molecule for nanoparticles. However, it is a suitable probe for sensitive detection of polymeric nanoparticles.