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Immobilization of Enzymes on PLGA Sub‐Micrometer Particles by Crosslinked Layer‐by‐Layer Deposition
Author(s) -
Sieber Sandro,
Siegrist Stefan,
Schwarz Stéphanie,
Porta Fabiola,
Schenk Susanne H.,
Huwyler Jörg
Publication year - 2017
Publication title -
macromolecular bioscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.924
H-Index - 105
eISSN - 1616-5195
pISSN - 1616-5187
DOI - 10.1002/mabi.201700015
Subject(s) - plga , dispersity , layer (electronics) , chemistry , immobilized enzyme , layer by layer , chemical engineering , nanotechnology , materials science , enzyme , polymer chemistry , biochemistry , nanoparticle , engineering
Enzyme immobilization is of high interest for industrial applications. However, immobilization may compromise enzyme activity or stability due to the harsh conditions which have to be applied. The authors therefore present a new and improved crosslinked layer‐by‐layer (cLbL) approach. Two different model enzymes (acid phosphatase and β‐galactosidase) are immobilized under mild conditions on biocompatible, monodisperse, sub‐micrometer poly(lactide‐ co ‐glycolide) (PLGA) particles. The resulting PLGA enzyme systems are characterized regarding their size, surface charge, enzyme activity, storage stability, reusability, and stability under various conditions such as changing pH and temperature. The developed and characterized cLbL protocol can be easily adapted to different enzymes. Potential future uses of the technology for biomedical applications are discussed. PLGA‐enzyme particles are therefore injected into the blood circulation of zebrafish embryos in order to demonstrate the in vivo stability and activity of the designed system.