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Designing Smart Materials with Recombinant Proteins
Author(s) -
Hollingshead Sydney,
Lin CharngYu,
Liu Julie C.
Publication year - 2017
Publication title -
macromolecular bioscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.924
H-Index - 105
eISSN - 1616-5195
pISSN - 1616-5187
DOI - 10.1002/mabi.201600554
Subject(s) - recombinant dna , protein design , chemistry , protein engineering , cleavage (geology) , modular design , chemical modification , protein structure , amino acid , biophysics , drug delivery , peptide sequence , biochemistry , enzyme , materials science , computer science , biology , organic chemistry , fracture (geology) , composite material , gene , operating system
Recombinant protein design allows modular protein domains with different functionalities and responsive behaviors to be easily combined. Inclusion of these protein domains can enable recombinant proteins to have complex responses to their environment (e.g., temperature‐triggered aggregation followed by enzyme‐mediated cleavage for drug delivery or pH‐triggered conformational change and self‐assembly leading to structural stabilization by adjacent complementary residues). These “smart” behaviors can be tuned by amino acid identity and sequence, chemical modifications, and addition of other components. A wide variety of domains and peptides have smart behavior. This review focuses on protein designs for self‐assembly or conformational changes due to stimuli such as shifts in temperature or pH.