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Inkjetting Plus Peroxidase‐Mediated Hydrogelation Produces Cell‐Laden, Cell‐Sized Particles with Suitable Characters for Individual Applications
Author(s) -
Sakai Shinji,
Yamamoto Yusuke,
Enkhtuul Gantumur,
Ueda Kohei,
Arai Kenichi,
Taya Masahito,
Nakamura Makoto
Publication year - 2017
Publication title -
macromolecular bioscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.924
H-Index - 105
eISSN - 1616-5195
pISSN - 1616-5187
DOI - 10.1002/mabi.201600416
Subject(s) - horseradish peroxidase , polymer , aqueous solution , chemistry , peroxidase , viability assay , cell , chemical engineering , piezoelectricity , nanotechnology , biophysics , polymer chemistry , materials science , organic chemistry , biochemistry , enzyme , composite material , biology , engineering
The authors report a method to prepare cell‐laden, cell‐sized microparticles from various materials suitable for individual applications. The method includes a piezoelectric inkjetting technology and a horseradish peroxidase (HRP)‐catalyzed crosslinking reaction. The piezoelectric inkjetting technology enables production of cell‐laden, cell‐sized (20–60 μm) droplets from a polymer aqueous solution. The HRP‐catalyzed crosslinking of the polymer in the ejected solution enables production of spherical microparticles from various materials. Superior cytocompatibility of the microencapsulation method is confirmed from the viability and growth profiles of normal murine mammary gland epithelial cells.