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Electrospun Lipid Binding Proteins Composite Nanofibers with Antibacterial Properties
Author(s) -
Tomaselli Simona,
Ramirez Diego Omar Sanchez,
Carletto Riccardo Andrea,
Varesano Alessio,
Vineis Claudia,
Zanzoni Serena,
Molinari Henriette,
Ragona Laura
Publication year - 2017
Publication title -
macromolecular bioscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.924
H-Index - 105
eISSN - 1616-5195
pISSN - 1616-5187
DOI - 10.1002/mabi.201600300
Subject(s) - nanofiber , electrospinning , chemistry , molecule , nanotechnology , biophysics , chemical engineering , materials science , polymer , organic chemistry , engineering , biology
Electrospinning is here used for the first time to prepare nanofibers including a host/guest complex in a keratin/poly(ethylene oxide) matrix. The host is a lipid binding protein and the guest is an insoluble bactericidal molecule, irgasan, bound within the protein internal cavity. The obtained nanofibers, characterized by scanning electron microscopy, exhibit excellent antibacterial activity toward Gram positive and negative bacteria, even with a moderate protein/irgasan cargo. Solution NMR studies, employed to provide molecular information on the cargo system, points to a micromolar affinity, compatible with both the electrospinning process and slow guest release. The versatility of the carrier protein, capable of interacting with a variety of druggable hydrophobic molecules, is exploitable for the development of innovative biomedical devices, whose properties can be tuned by the selected guest.