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Gelatin Porous Scaffolds as Delivery Systems of Calcium Alendronate
Author(s) -
Panzavolta Silvia,
Torricelli Paola,
Casolari Sonia,
Parrilli Annapaola,
Amadori Sofia,
Fini Milena,
Bigi Adriana
Publication year - 2017
Publication title -
macromolecular bioscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.924
H-Index - 105
eISSN - 1616-5195
pISSN - 1616-5187
DOI - 10.1002/mabi.201600272
Subject(s) - gelatin , glutaraldehyde , osteoclast , osteoblast , chemistry , calcium , drug delivery , porosity , biophysics , in vitro , biomedical engineering , chemical engineering , biochemistry , chromatography , organic chemistry , medicine , engineering , biology
The systemic administration of bisphosphonates (BPs) for the treatment of metabolic diseases characterized by abnormal bone loss suffers from several adverse side effects, which can be reduced by implementation of alternative modes of administration. In this work, glutaraldehyde cross‐linked gelatin scaffolds are proposed as delivery systems of calcium alendronate monohydrate (CaAL•H 2 O). The 3D highly porous scaffolds display a relevant interconnected porosity (>94%), independently from CaAL•H 2 O content (0, 3, and 6 wt%). At variance, pore size varies with composition. The relative increase of the number of smaller pores on increasing BP content is in agreement with the parallel significant increase of the compressive modulus and collapse strength. The scaffolds exhibit a sustained CaAL•H 2 O release profile, and a significant amount of the drug is retained in the scaffolds even after 14 d. In vitro tests are carried out using cocultures of osteoblast (OB) and osteoclast (OC). The evaluation of differentiation markers is performed both on the supernatants of cell culture and by means of quantitative polymerase chain reaction. The results indicate that BP containing scaffolds support osteoblast proliferation and differentiation, whereas they inhibit osteoclast viability and activity, displaying a promising beneficial role on bone repair processes.