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Generation of Large‐Scale DNA Hydrogels with Excellent Blood and Cell Compatibility
Author(s) -
Stoll Heidi,
Steinle Heidrun,
Stang Katharina,
Kunnakattu Silju,
Scheideler Lutz,
Neumann Bernd,
Kurz Julia,
Degenkolbe Ilka,
Perle Nadja,
Schlensak Christian,
Wendel Hans Peter,
AvciAdali Meltem
Publication year - 2017
Publication title -
macromolecular bioscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.924
H-Index - 105
eISSN - 1616-5195
pISSN - 1616-5187
DOI - 10.1002/mabi.201600252
Subject(s) - self healing hydrogels , biomaterial , hemolysis , chemistry , tissue engineering , biomedical engineering , dna , nanotechnology , biophysics , materials science , polymer chemistry , biochemistry , immunology , biology , medicine
Hemocompatibility and cytocompatibility of biomaterials codetermine the success of tissue engineering applications. DNA, the natural component of our cells, is an auspicious biomaterial for the generation of designable scaffolds with tailorable characteristics. In this study, a combination of rolling circle amplification and multiprimed chain amplification is used to generate hydrogels at centimeter scale consisting solely of DNA. Using an in vitro rotation model and fresh human blood, the reaction of the hemostatic system on DNA hydrogels is analyzed. The measurements of hemolysis, platelets activation, and the activation of the complement, coagulation, and neutrophils using enzyme‐linked immunosorbent assays demonstrate excellent hemocompatibility. In addition, the cytocompatibility of the DNA hydrogels is tested by indirect contact (agar diffusion tests) and material extract experiments with L929 murine fibroblasts according to the ISO 10993‐5 specifications and no negative impact on the cell viability is detected. These results indicate the promising potential of DNA hydrogels as biomaterials for versatile applications in the field of regenerative medicine.