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The Synergistic Effect of Cationic Moieties and GRGDSF‐Peptides in Hydrogels on Neural Stem Cell Behavior
Author(s) -
Sallouh Muhammad,
Jarocki Marvin,
Sallouh Omar,
Degen Patrick,
Faissner Andreas,
Weberskirch Ralf
Publication year - 2017
Publication title -
macromolecular bioscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.924
H-Index - 105
eISSN - 1616-5195
pISSN - 1616-5187
DOI - 10.1002/mabi.201600178
Subject(s) - self healing hydrogels , cationic polymerization , chemistry , cell adhesion , serine , peptide , glycine , residue (chemistry) , embryonic stem cell , aspartic acid , neural cell adhesion molecule , amino acid , biophysics , polymer chemistry , cell , biochemistry , biology , enzyme , gene
This article reports the behavior of embryonic neural stem cells on a hydrogel that combines cationic, non‐specific cell adhesion motifs with glycine‐arginine‐glycine‐aspartic acid‐serine‐phenylalanine (GRGDSF)‐peptides as specific cell adhesion moieties. Therefore, three hydrogels are prepared by free radical polymerization that contains either a GRGDSF‐peptide residue ( P1 ), amino ethylmethacrylate as a cationic residue ( P2 ), or a combination of both motifs ( P3 ). For each gel, cross linker concentrations of 8 mol% is used to have a comparable gel stiffness of 8–9 kPa. The cell experiments indicate a synergistic effect of the non‐specific, cationic residues, and the specific GRGDSF‐peptides on embryonic neural stem cell behavior that is especially pronounced in the cell adhesion experiments by more than doubling the number of cells after 72 h when comparing P3 with P2 and is less pronounced in the proliferation and differentiation experiments.