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RGDS‐ and SIKVAVS‐Modified Superporous Poly(2‐hydroxyethyl methacrylate) Scaffolds for Tissue Engineering Applications
Author(s) -
Macková Hana,
Plichta Zdeněk,
Proks Vladimír,
Kotelnikov Ilya,
Kučka Jan,
Hlídková Helena,
Horák Daniel,
Kubinová Šárka,
Jiráková Klára
Publication year - 2016
Publication title -
macromolecular bioscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.924
H-Index - 105
eISSN - 1616-5195
pISSN - 1616-5187
DOI - 10.1002/mabi.201600159
Subject(s) - methacrylate , tissue engineering , scaffold , 2 hydroxyethyl methacrylate , mesenchymal stem cell , chemistry , copolymer , linker , polymer chemistry , biomedical engineering , materials science , polymer , organic chemistry , microbiology and biotechnology , medicine , computer science , biology , operating system
Three‐dimensional hydrogel supports for mesenchymal and neural stem cells (NSCs) are promising materials for tissue engineering applications such as spinal cord repair. This study involves the preparation and characterization of superporous scaffolds based on a copolymer of 2‐hydroxyethyl and 2‐aminoethyl methacrylate (HEMA and AEMA) crosslinked with ethylene dimethacrylate. Ammonium oxalate is chosen as a suitable porogen because it consists of needle‐like crystals, allowing their parallel arrangement in the polymerization mold. The amino group of AEMA is used to immobilize RGDS and SIKVAVS peptide sequences with an N ‐γ‐maleimidobutyryloxy succinimide ester linker. The amount of the peptide on the scaffold is determined using 125 I radiolabeled SIKVAVS. Both RGDS‐ and SIKVAVS‐modified poly(2‐hydroxyethyl methacrylate) scaffolds serve as supports for culturing human mesenchymal stem cells (MSCs) and human fetal NSCs. The RGDS sequence is found to be better for MSC and NSC proliferation and growth than SIKVAVS.