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Electrospun Gelatin Fibers with a Multiple Release of Antibiotics Accelerate Dermal Regeneration in Infected Deep Burns
Author(s) -
Chen Jianmei,
Liu Zongguang,
Chen Maohua,
Zhang Hong,
Li Xiaohong
Publication year - 2016
Publication title -
macromolecular bioscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.924
H-Index - 105
eISSN - 1616-5195
pISSN - 1616-5187
DOI - 10.1002/mabi.201600108
Subject(s) - gelatin , electrospinning , staphylococcus epidermidis , chemistry , ciprofloxacin , regeneration (biology) , antibiotics , wound healing , gentamicin sulfate , controlled release , biomedical engineering , microbiology and biotechnology , staphylococcus aureus , bacteria , gentamicin , surgery , pharmacology , polymer , medicine , biochemistry , organic chemistry , biology , genetics
Electrospun fibers of hydrophilic polymers meet challenges in a rapid degradation of fiber matrices and discharge of antibiotics to comply with requirements of infection control as a dermal regeneration template. In the current study, a pH conversion process is initially developed to ensure fluent electrospinning, an efficient in situ cross‐linking of electrospun gelatin fibers with oxidized alginate and simultaneous loading of gentamicin sulfate (GS) and hydrophobic ciprofloxacin into fibers. The dual drug‐loaded fibers indicate a complete release of GS during 6 d and a sustained release of ciprofloxacin for over three weeks, and the antibiotics release indicates significant growth inhibitions on Pseudomonas aeruginosa and Staphylococcus epidermidis . The wound healing efficacy is evaluated on a deep burn model infected with 10 8 CFU of P. aeruginosa . Compared with fibers with loaded individual drugs, the concomitant release of GS and ciprofloxacin significantly reduces the bacteria numbers in wound and livers, at around 2.30 × 10 5 and 1.25 × 10 3 CFU after 3 d, respectively. The wound re‐epithelization, blood vessel formation, collagen deposition, and tissue remodeling process are accelerated with a complete healing observed after 21 d. This study provides a feasible strategy to design cross‐linked hydrophilic fibers with an extended drug release for biomedical applications.