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Tuning the Surface of Nanoparticles: Impact of Poly(2‐ethyl‐2‐oxazoline) on Protein Adsorption in Serum and Cellular Uptake
Author(s) -
Koshkina Olga,
Westmeier Dana,
Lang Thomas,
Bantz Christoph,
Hahlbrock Angelina,
Würth Christian,
ReschGenger Ute,
Braun Ulrike,
Thiermann Raphael,
Weise Christoph,
Eravci Murat,
Mohr Benjamin,
Schlaad Helmut,
Stauber Roland H.,
Docter Dominic,
Bertin Annabelle,
Maskos Michael
Publication year - 2016
Publication title -
macromolecular bioscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.924
H-Index - 105
eISSN - 1616-5195
pISSN - 1616-5187
DOI - 10.1002/mabi.201600074
Subject(s) - nanoparticle , nanomedicine , protein adsorption , adsorption , chemistry , surface modification , peg ratio , ethylene glycol , biodistribution , dynamic light scattering , biomolecule , chemical engineering , hydrodynamic radius , chromatography , nanotechnology , materials science , organic chemistry , biochemistry , finance , engineering , economics , in vitro
Due to the adsorption of biomolecules, the control of the biodistribution of nanoparticles is still one of the major challenges of nanomedicine. Poly(2‐ethyl‐2‐oxazoline) (PEtOx) for surface modification of nanoparticles is applied and both protein adsorption and cellular uptake of PEtOxylated nanoparticles versus nanoparticles coated with poly(ethylene glycol) (PEG) and non‐coated positively and negatively charged nanoparticles are compared. Therefore, fluorescent poly(organosiloxane) nanoparticles of 15 nm radius are synthesized, which are used as a scaffold for surface modification in a grafting onto approach. With multi‐angle dynamic light scattering, asymmetrical flow field‐flow fractionation, gel electrophoresis, and liquid chromatography‐mass spectrometry, it is demonstrated that protein adsorption on PEtOxylated nanoparticles is extremely low, similar as on PEGylated nanoparticles. Moreover, quantitative microscopy reveals that PEtOxylation significantly reduces the non‐specific cellular uptake, particularly by macrophage‐like cells. Collectively, studies demonstrate that PEtOx is a very effective alternative to PEG for stealth modification of the surface of nanoparticles.