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In Vivo Osteogenic Differentiation of Human Dental Pulp Stem Cells Embedded in an Injectable In Vivo‐Forming Hydrogel
Author(s) -
Jang Ja Yong,
Park Seung Hun,
Park Ji Hoon,
Lee Bo Keun,
Yun JeongHo,
Lee Bong,
Kim Jae Ho,
Min Byoung Hyun,
Kim Moon Suk
Publication year - 2016
Publication title -
macromolecular bioscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.924
H-Index - 105
eISSN - 1616-5195
pISSN - 1616-5187
DOI - 10.1002/mabi.201600001
Subject(s) - in vivo , dental pulp stem cells , self healing hydrogels , chemistry , osteopontin , osteocalcin , tissue engineering , biomedical engineering , osteonectin , ex vivo , staining , microbiology and biotechnology , alkaline phosphatase , in vitro , pathology , biochemistry , biology , medicine , immunology , organic chemistry , enzyme
In this study, human dental pulp stem cells (hDPSCs) are examined as a cellular source for bone tissue engineering using an in vivo‐forming hydrogel. The hDPSCs are easily harvested in large quantities from extracted teeth. The stemness of harvested hDPSCs indicates their relative tolerance to ex vivo manipulation in culture. The in vitro osteogenic differentiation of hDPSCs is characterized using Alizarin Red S (ARS), von Kossa (VK), and alkaline phosphatase (ALP) staining. The solution of hDPSCs and a methoxy polyethylene glycol‐polycaprolactone block copolymer (PC) is easily prepared by simple mixing at room temperature and in no more than 10 s it forms in vivo hydrogels after subcutaneous injection into rats. In vivo osteogenic differentiation of hDPSCs in the in vivo‐forming hydrogel is confirmed by micro‐computed tomography (CT), histological staining, and gene expression. Micro‐CT analysis shows evidence of significant tissue‐engineered bone formation in hDPSCs‐loaded hydrogel in the presence of osteogenic factors. Differentiated osteoblasts in in vivo‐forming hydrogel are identified by ARS and VK staining and are found to exhibit characteristic expression of genes like osteonectin, osteopontin, and osteocalcin. In conclusion, hDPSCs embedded in an in vivo‐forming hydrogel may provide benefits as a noninvasive formulation for bone tissue engineering applications.