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A Tailor‐Made Synthetic Polymer for Cell Encapsulation: Design Rationale, Synthesis, Chemical–Physics and Biological Characterizations
Author(s) -
Gerges Irini,
Tamplenizza Margherita,
Rossi Eleonora,
Tocchio Alessandro,
Martello Federico,
Recordati Camilla,
Kumar Deepak,
Forsyth Nicholas R.,
Liu Yang,
Lenardi Cristina
Publication year - 2016
Publication title -
macromolecular bioscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.924
H-Index - 105
eISSN - 1616-5195
pISSN - 1616-5187
DOI - 10.1002/mabi.201500386
Subject(s) - methacrylamide , biocompatibility , polymer , methacrylate , cell encapsulation , self healing hydrogels , materials science , polymer chemistry , chemistry , nanotechnology , chemical engineering , polymerization , copolymer , organic chemistry , acrylamide , engineering
This study presents a custom‐made in situ gelling polymeric precursor for cell encapsulation. Composed of poly((2‐hydroxyethyl)methacrylate‐co‐(3‐aminopropyl)methacrylamide) (P(HEMA‐co‐APM) mother backbone and RGD‐mimicking poly(amidoamine) (PAA) moiteis, the comb‐like structured polymeric precursor is tailored to gather the advantages of the two families of synthetic polymers, i.e., the good mechanical integrity of PHEMA‐based polymers and the biocompatibility and biodegradability of PAAs. The role of P(HEMA‐co‐APM) in the regulation of the chemico‐physical properties of P(HEMA‐co‐APM)/PAA hydrogels is thoroughly investigated. On the basis of obtained results, namely the capability of maintaining vital NIH3T3 cell line in vitro for 2 d in a 3D cell culture, the in vivo biocompatibility in murine model for 16 d, and the ability of finely tuning mechanical properties and degradation kinetics, it can be assessed that P(HEMA‐co‐APM)/PAAs offer a cost‐effective valid alternative to the so far studied natural polymer‐based systems for cell encapsulation.