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Three‐Layered PCL Grafts Promoted Vascular Regeneration in a Rabbit Carotid Artery Model
Author(s) -
Wang Kai,
Zheng Wenting,
Pan Yiwa,
Ma Shaoyang,
Guan Yong,
Liu Ruming,
Zhu Meifeng,
Zhou Xin,
Zhang Jun,
Zhao Qiang,
Zhu Yan,
Wang Lianyong,
Kong Deling
Publication year - 2016
Publication title -
macromolecular bioscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.924
H-Index - 105
eISSN - 1616-5195
pISSN - 1616-5187
DOI - 10.1002/mabi.201500355
Subject(s) - regeneration (biology) , elastin , electrospinning , chemistry , in vivo , biomedical engineering , infiltration (hvac) , carotid arteries , anatomy , materials science , pathology , surgery , microbiology and biotechnology , medicine , biology , polymer , composite material , organic chemistry
In this study, a three layered poly (ε‐caprolactone) (PCL) graft (tPCL) was fabricated by electrospinning PCL and electrospraying poly (ethylene oxide) (PEO), which has a thin dense inner layer, a loose middle layer, and a dense outer layer. Regular PCL grafts (rPCL) with only a dense layer were used as control. In vivo evaluation was performed in rabbit carotid artery. Enhanced cell infiltration, rapid regeneration of endothelium and smooth muscle layers, and increased elastin deposition were observed within the tPCL graft wall. After 3 months, tPCL grafts showed faster PCL degradation than the rPCL grafts. Infiltrated macrophages in the tPCL grafts secreted higher level of monocyte chemoattractant protein‐1 (MCP‐1) and vascular endothelial growth factor (VEGF) which enhanced vascular regeneration. In conclusion, the tPCL graft may be a useful vascular prosthesis and worth for further investigation.