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Two‐Phase Electrospinning to Incorporate Polyelectrolyte Complexes and Growth Factors into Electrospun Chitosan Nanofibers
Author(s) -
Place Laura W.,
Sekyi Maria,
Taussig Julia,
Kipper Matt J.
Publication year - 2016
Publication title -
macromolecular bioscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.924
H-Index - 105
eISSN - 1616-5195
pISSN - 1616-5187
DOI - 10.1002/mabi.201500288
Subject(s) - electrospinning , nanofiber , chitosan , vinyl alcohol , emulsion , chemical engineering , polyelectrolyte , fibroblast growth factor , chemistry , polymer , nanoparticle , polymer chemistry , phase (matter) , aqueous solution , materials science , organic chemistry , biochemistry , receptor , engineering
Growth factors are potent signaling proteins for tissue engineering, but they are susceptible to loss of activity when exposed to solvents used for polymer processing. This work explores preservation of fibroblast growth factor‐2 (FGF‐2) activity in chitosan nanofibers using two‐phase electrospinning via a compound coaxial needle and from a water‐in‐oil emulsion FGF‐2 in aqueous poly(vinyl alcohol) is added on either the inside (A/O) or the outside (O/A) of an organic chitosan phase, using the compound needle. FGF‐2 is further stabilized by complexation to heparin‐based nanoparticles. The emulsion method does not result in detectable incorporation of FGF‐2. The A/O fibers incorporate the highest amount of FGF‐2. Nanoparticle‐stabilized FGF‐2 in A/O nanofibers is most active toward bone‐marrow stromal cells.