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Development of Non‐Cell Adhesive Vascular Grafts Using Supramolecular Building Blocks
Author(s) -
van Almen Geert C.,
Talacua Hanna,
Ippel Bastiaan D.,
Mollet Björne B.,
Ramaekers Mellany,
Simonet Marc,
Smits Anthal I. P. M.,
Bouten Carlijn V. C.,
Kluin Jolanda,
Dankers Patricia Y. W.
Publication year - 2016
Publication title -
macromolecular bioscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.924
H-Index - 105
eISSN - 1616-5195
pISSN - 1616-5187
DOI - 10.1002/mabi.201500278
Subject(s) - polycaprolactone , supramolecular chemistry , ethylene glycol , tissue engineering , adhesive , cell adhesion , supramolecular polymers , peg ratio , materials science , in situ , biomedical engineering , adhesion , chemistry , polymer chemistry , polymer , nanotechnology , composite material , organic chemistry , molecule , medicine , finance , layer (electronics) , economics
Cell‐free approaches to in situ tissue engineering require materials that are mechanically stable and are able to control cell‐adhesive behavior upon implantation. Here, the development of mechanically stable grafts with non‐cell adhesive properties via a mix‐and‐match approach using ureido‐pyrimidinone (UPy)‐modified supramolecular polymers is reported. Cell adhesion is prevented in vitro through mixing of end‐functionalized or chain‐extended UPy‐polycaprolactone (UPy‐PCL or CE‐UPy‐PCL, respectively) with end‐functionalized UPy‐poly(ethylene glycol) (UPy‐PEG) at a ratio of 90:10. Further characterization reveals intimate mixing behavior of UPy‐PCL with UPy‐PEG, but poor mechanical properties, whereas CE‐UPy‐PCL scaffolds are mechanically stable. As a proof‐of‐concept for the use of non‐cell adhesive supramolecular materials in vivo, electrospun vascular scaffolds are applied in an aortic interposition rat model, showing reduced cell infiltration in the presence of only 10% of UPy‐PEG. Together, these results provide the first steps toward advanced supramolecular biomaterials for in situ vascular tissue engineering with control over selective cell capturing.