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Tailored Fringed Platforms Produced by Laser Interference for Aligned Neural Cell Growth
Author(s) -
Peláez Ramón J.,
GonzálezMayorga Ankor,
Gutiérrez María C.,
GarcíaRama Concepción,
Afonso Carmen N.,
Serrano María C.
Publication year - 2016
Publication title -
macromolecular bioscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.924
H-Index - 105
eISSN - 1616-5195
pISSN - 1616-5187
DOI - 10.1002/mabi.201500253
Subject(s) - neurite , neural cell , adhesion , nanotechnology , cell adhesion , neural stem cell , materials science , progenitor cell , laser , proof of concept , substrate (aquarium) , cell , chemistry , microbiology and biotechnology , computer science , biology , stem cell , in vitro , optics , physics , biochemistry , operating system , ecology , composite material
Ordering neural cells is of interest for the development of neural interfaces. The aim of this work is to demonstrate an easy‐to‐use, versatile, and cost/time effective laser‐based approach for producing platforms that promote oriented neural growth. We use laser interferometry to generate fringed channels with topography on partially reduced graphene oxide layers as a proof‐of‐concept substrate. We study cell adhesion, morphology, viability, and differentiation in cultures of embryonic neural progenitor cells on platforms with a 9.4 μm period. Results evidence that fringed platforms significantly promote neurite alignment (≈50% at 6 d), while preserving viability and neural differentiation.
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