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Protein‐Containing Multilayer Capsules by Templating on Mesoporous CaCO 3 Particles: POST‐ and PRE‐Loading Approaches
Author(s) -
Balabushevich Nadezhda G.,
Lopez de Guerenu Anna V.,
Feoktistova Natalia A.,
Skirtach Andre G.,
Volodkin Dmitry
Publication year - 2016
Publication title -
macromolecular bioscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.924
H-Index - 105
eISSN - 1616-5195
pISSN - 1616-5187
DOI - 10.1002/mabi.201500243
Subject(s) - mesoporous material , chemistry , chemical engineering , hydrolysis , macromolecule , dextran , adsorption , protein adsorption , materials science , chromatography , organic chemistry , biochemistry , catalysis , engineering
Encapsulation of model proteins (catalase, insulin, aprotinin) into multilayer dextran sulphate/protamin capsules by templating on CaCO 3 microparticles is investigated employing: (i) PRE‐loading into CaCO 3 particles by adsorption or co‐synthesis and (ii) POST‐loading into performed capsules. Protein encapsulation is governed by both its size and electrostatic interactions with the carbonate microparticles and multilayer shell. PRE‐loading enables improved encapsulation compared to POST‐loading (catalase content in capsules 630 and 70 mg · g −1 ). Bioactivity of encapsulated protein is not affected by interaction with multilayers but may be reduced at slightly alkaline pH due to CaCO 3 hydrolysis. This study might help to successfully encapsulate fragile bio‐macromolecules into multilayer capsules.