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Efficient pDNA Delivery Using Cationic 2‐Hydroxypropyl‐β‐Cyclodextrin Pluronic‐Based Polyrotaxanes
Author(s) -
Badwaik Vivek,
Mondjinou Yawo,
Kulkarni Aditya,
Liu Linjia,
Demoret Asher,
Thompson David H.
Publication year - 2016
Publication title -
macromolecular bioscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.924
H-Index - 105
eISSN - 1616-5195
pISSN - 1616-5187
DOI - 10.1002/mabi.201500220
Subject(s) - transfection , cationic polymerization , chemistry , poloxamer , cyclodextrin , biophysics , drug delivery , gene delivery , polymer chemistry , nuclear chemistry , copolymer , biochemistry , organic chemistry , polymer , biology , gene
A family of cationic Pluronic‐based polyrotaxanes (PR + ), threaded with 2‐hydroxypropyl‐β‐cyclodextrin (HPCD), was synthesized for pDNA delivery into multiple cell lines. All PR + formed highly stable, positively charged pDNA complexes that were < 250 nm in diameter. The cellular uptake and pDNA transfection efficiencies of the PR + :pDNA complexes was enhanced relative to the commercial transfection standards L2K and bPEI, while displaying similar or lower toxicity profiles. Charge density and threading efficiency of the PR + agent significantly influenced the colloidal stability and physical properties of the complexes, which impacted their intracellular transfection efficiencies. Taken together, our results suggest that HPCD: Pluronic PR + can be used as potent vectors for pDNA‐based therapeutics.