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EGFP‐Based Protein Nanoparticles with Cell‐Penetrating Peptide for Efficient siRNA Delivery
Author(s) -
Guan Xingang,
Hu Xiuli,
Cui Fengchao,
Li Yunqi,
Jing Xiabing,
Xie Zhigang
Publication year - 2015
Publication title -
macromolecular bioscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.924
H-Index - 105
eISSN - 1616-5195
pISSN - 1616-5187
DOI - 10.1002/mabi.201500163
Subject(s) - nanocarriers , nanoparticle , chemistry , cell penetrating peptide , nucleic acid , small interfering rna , peptide , green fluorescent protein , biophysics , gene silencing , nanotechnology , transfection , microbiology and biotechnology , biochemistry , materials science , biology , gene
Development of an innovative nucleic acid nanocarriers still represents a challenge. In this study, we develop a protein nanoparticle (H6‐TatEGFP) and examine its siRNA condensing activity. Gel retardation assay show that protein nanoparticle can condense siRNA into stable nanoparticle/siRNA complexes. UsingCy3‐labelled siRNA, we also evaluate siRNA transport characteristic of protein nanoparticles in tumor cells, the results indicate that H6‐TatEGFP nanoparticle may be a potential nanocarrier for siRNA in tumor cells.
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