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Cellular Response to Linear and Branched Poly(acrylic acid)
Author(s) -
Whitty Elizabeth G.,
Maniego Alison R.,
Bentwitch Sharon A.,
Guillaneuf Yohann,
Jones Mark R.,
Gaborieau Marianne,
Castignolles Patrice
Publication year - 2015
Publication title -
macromolecular bioscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.924
H-Index - 105
eISSN - 1616-5195
pISSN - 1616-5187
DOI - 10.1002/mabi.201500153
Subject(s) - acrylic acid , chemistry , cytotoxicity , capillary electrophoresis , mtt assay , acrylate , viability assay , fluorescence microscope , confocal microscopy , bradford protein assay , fluorescence , biophysics , intracellular , microbiology and biotechnology , biochemistry , cell , chromatography , in vitro , polymer , monomer , organic chemistry , biology , physics , quantum mechanics
Poly(acrylic acid‐ co ‐sodium acrylate) (PNaA) is a pH‐responsive polymer with potential in anticancer drug delivery. The cytotoxicity and intracellular effects of 3‐arm star, hyperbranched and linear PNaA were investigated with L1210 progenitor leukemia cells and L6 myoblast cells. Free solution capillary electrophoresis demonstrated interactions of PNaA with serum proteins. In a 72 h MTT assay most PNaAs exhibited a IC 50 between 7 and 14 mmol L −1 , showing that precipitation may be a sufficient purification for PNaA dilute solutions. Dialyzed 3‐arm star and hyperbranched PNaA caused an increase in L6 cell viability, challenging the suitability of MTT as cytotoxicity assay for PNaA. Fluorescent confocal microscopy revealed merging of cellular lipids after exposure to PNaA, likely caused by serum starvation.