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Fluorescent Phosphorus Dendrimer as a Spectral Nanosensor for Macrophage Polarization and Fate Tracking in Spinal Cord Injury
Author(s) -
Shakhbazau Antos,
Mishra Manoj,
Chu TakHo,
Brideau Craig,
Cummins Karen,
Tsutsui Shigeki,
Shcharbin Dzmitry,
Majoral JeanPierre,
Mignani Serge,
BlanchardDesce Mireille,
Bryszewska Maria,
Yong V. Wee,
Stys Peter K.,
van Minnen Jan
Publication year - 2015
Publication title -
macromolecular bioscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.924
H-Index - 105
eISSN - 1616-5195
pISSN - 1616-5187
DOI - 10.1002/mabi.201500150
Subject(s) - dendrimer , spinal cord injury , chemistry , fluorescence , biophysics , macrophage polarization , intracellular , microbiology and biotechnology , macrophage , spinal cord , nanotechnology , materials science , in vitro , biology , biochemistry , neuroscience , physics , quantum mechanics
Dendrimers and dendriplexes, highly branched synthetic macromolecules, have gained popularity as new tools for a variety of nanomedicine strategies due to their unique structure and properties. We show that fluorescent phosphorus dendrimers are well retained by bone marrow‐derived macrophages and exhibit robust spectral shift in its emission in response to polarization conditions. Fluorescence properties of this marker can also assist in identifying macrophage presence and phenotype status at different time points after spinal cord injury. Potential use of a single dendrimer compound as a drug/siRNA carrier and phenotype‐specific cell tracer offers new avenues for enhanced cell therapies combined with monitoring of cell fate and function in spinal cord injury.