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Crosslinked Polypropylenimine Dendrimers With Bioreducible Linkages for Gene Delivery Systems and Their Reductive Degradation Behaviors
Author(s) -
Lee Gyeong Jin,
Ryu Kitae,
Kim Kyunghwan,
Choi Jiyeong,
Kim Taeil
Publication year - 2015
Publication title -
macromolecular bioscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.924
H-Index - 105
eISSN - 1616-5195
pISSN - 1616-5187
DOI - 10.1002/mabi.201500141
Subject(s) - cytotoxicity , dendrimer , glutathione , reactive oxygen species , chemistry , gene delivery , transfection , degradation (telecommunications) , oxygen , oxidative stress , polymer , biophysics , biochemistry , gene , in vitro , organic chemistry , biology , enzyme , telecommunications , computer science
Crosslinked bioreducible polypropylenimine‐cystaminebisacrylamides (PPI‐CBAs) were synthesized for gene delivery systems. They formed nano‐sized polyplexes with high stability even in reducing condition probably due to the re‐crosslinking. PPI‐CBAs displayed high transfection efficiency comparable to PEI25k in serum condition. However, cytotoxicity increase, glutathione (GSH) decrease, and reactive oxygen species (ROS) increase were observed in cells with the elevation of PPI‐CBAs concentration and crosslinking degree. These results suggest that the cytotoxicity of bioreducible polymers may be closely related with their structures and that reduction of GSH by degradation of re‐crosslinked bioreducible polymers and the following increase of ROS may induce cytotoxicity by oxidative stress.