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A New Hyaluronic Acid Derivative Obtained from Atom Transfer Radical Polymerization as a siRNA Vector for CD44 Receptor Tumor Targeting
Author(s) -
Palumbo Fabio Salvatore,
Bavuso Volpe Antonella,
Bongiovì Flavia,
Pitarresi Giovanna,
Giammona Gaetano
Publication year - 2015
Publication title -
macromolecular bioscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.924
H-Index - 105
eISSN - 1616-5195
pISSN - 1616-5187
DOI - 10.1002/mabi.201500129
Subject(s) - atom transfer radical polymerization , chemistry , polymerization , hyaluronic acid , polymer chemistry , methacrylate , radical polymerization , cationic polymerization , dynamic light scattering , cd44 , monomer , biophysics , in vitro , polymer , organic chemistry , materials science , biochemistry , nanotechnology , biology , nanoparticle , genetics
Two derivatives of hyaluronic acid (HA) have been synthesized by atom transfer radical polymerization (ATRP), starting from an ethylenediamino HA derivative (HA‐EDA) and by using diethylaminoethyl methacrylate (DEAEMA) as a monomer for polymerization. Both samples, indicated as HA‐EDA‐pDEAEMA a and b, are able to condense siRNA, as determined by gel retardation assay and resulting complexes show a size and a zeta potential value dependent on polymerization number, as determined by dynamic light scattering measurements. In vitro studies performed on HCT 116 cell line, that over express CD44 receptor, demonstrate a receptor mediated uptake of complexes, regardless of their surface charge.