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Advanced Evaluation Strategies for Protein‐Imprinted Polymer Nanobeads
Author(s) -
Pluhar Bettina,
Mizaikoff Boris
Publication year - 2015
Publication title -
macromolecular bioscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.924
H-Index - 105
eISSN - 1616-5195
pISSN - 1616-5187
DOI - 10.1002/mabi.201500106
Subject(s) - molecularly imprinted polymer , chemistry , selectivity , sodium dodecyl sulfate , protease , combinatorial chemistry , gel electrophoresis , polymer , chromatography , biochemistry , organic chemistry , enzyme , catalysis
Molecularly imprinted polymers (MIPs) are synthetic affinity matrices capable of selective binding a specific target molecule. A strategy for competitive selectivity studies is developed providing information on the selective binding properties of MIPs in complex matrices. Batch rebinding experiments entail the target protease, two other proteins, and MIP nanobeads. The protease is inhibited by addition of pepstatin thus quenching the degradation of the other proteins. The proteins are analyzed via sodium dodecyl sulfate‐polyacrylamide gel electrophoresis. The relevance of competitive selectivity studies for the evaluation of MIP performance is further emphasized by comparison to single protein rebinding experiments.