Premium
Biodegradable Nanotopography Combined with Neurotrophic Signals Enhances Contact Guidance and Neuronal Differentiation of Human Neural Stem Cells
Author(s) -
Yang Kisuk,
Park Esther,
Lee Jong Seung,
Kim IlSun,
Hong Kwonho,
Park Kook In,
Cho SeungWoo,
Yang Hee Seok
Publication year - 2015
Publication title -
macromolecular bioscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.924
H-Index - 105
eISSN - 1616-5195
pISSN - 1616-5187
DOI - 10.1002/mabi.201500080
Subject(s) - neurite , nanotopography , plga , neural stem cell , chemistry , microbiology and biotechnology , cellular differentiation , nanotechnology , neural tissue engineering , cell adhesion , neurotrophic factors , neurotrophin , biophysics , stem cell , materials science , cell , biochemistry , biology , regeneration (biology) , nanoparticle , in vitro , gene , receptor
Biophysical cues provided by nanotopographical surfaces have been used as stimuli to guide neurite extension and regulate neural stem cell (NSC) differentiation. Here, we fabricated biodegradable polymer substrates with nanoscale topography for enhancing human NSC (hNSC) differentiation and guided neurite outgrowth. The substrate was constructed from biodegradable poly(lactic‐co‐glycolic acid) (PLGA) using solvent‐assisted capillary force lithography. We found that precoating with 3,4‐dihydroxy‐ l ‐phenylalanine (DOPA) facilitated the immobilization of poly‐ l ‐lysine and fibronectin on PLGA substrates via bio‐inspired catechol chemistry. The DOPA‐coated nanopatterned substrates directed cellular alignment along the patterned grooves by contact guidance, leading to enhanced focal adhesion, skeletal protein reorganization, and neuronal differentiation of hNSCs as indicated by highly extended neurites from cell bodies and increased expression of neuronal markers (Tuj1 and MAP2). The addition of nerve growth factor further enhanced neuronal differentiation of hNSCs, indicating a synergistic effect of biophysical and biochemical cues on NSC differentiation. These bio‐inspired PLGA nanopatterned substrates could potentially be used as implantable biomaterials for improving the efficacy of hNSCs in treating neurodegenerative diseases.