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Substantial Differentiation of Human Neural Stem Cells Into Motor Neurons on a Biomimetic Polyurea
Author(s) -
Yun Donghwa,
Lee Young M.,
Laughter Melissa R.,
Freed Curt R.,
Park Daewon
Publication year - 2015
Publication title -
macromolecular bioscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.924
H-Index - 105
eISSN - 1616-5195
pISSN - 1616-5187
DOI - 10.1002/mabi.201500066
Subject(s) - polyurea , neural stem cell , scaffold , spinal cord injury , stem cell , chemistry , cellular differentiation , nanotechnology , neuroscience , biomedical engineering , materials science , microbiology and biotechnology , spinal cord , biology , coating , medicine , biochemistry , gene
To find the first restorative treatment for spinal cord injury (SCI), researchers have focused on stem cell therapies. However, one obstacle is the lack of an implantable cell scaffold that can support efficient motor neuron (MN) differentiation and proliferation. We aimed to overcome this through the use of an RGD functionalized novel biomimetic polyurea, optimized to encourage efficient differentiation of MNs. Images taken after 14‐days showed increased differentiation (∼40%) of hNSCs into MNs as well as increased cell count on the biomimetic polymer compared to PDL‐Laminin coating, indicating that the RGD‐polyurea provides a favorable microenvironment for hNSC survival, having promising implications for future SCI therapies.