Premium
Cover Picture: Macromol. Biosci. 10/2014
Author(s) -
Heller Philipp,
Birke Alexander,
Huesmann David,
Weber Benjamin,
Fischer Karl,
ReskeKunz Angelika,
Bros Matthias,
Barz Matthias
Publication year - 2014
Publication title -
macromolecular bioscience
Language(s) - English
Resource type - Reports
SCImago Journal Rank - 0.924
H-Index - 105
eISSN - 1616-5195
pISSN - 1616-5187
DOI - 10.1002/mabi.201470035
Subject(s) - sarcosine , transfection , chemistry , copolymer , peg ratio , in vitro , microbiology and biotechnology , biophysics , polymer chemistry , biochemistry , amino acid , polymer , biology , organic chemistry , glycine , finance , economics , gene
Front Cover: A series of well‐defined polypeptide‐polypeptoid block copolymers based on the body's own amino acids sarcosine and lysine are prepared by K. Fischer, M. Bros, M. Barz, and co‐workers on page 1380 by ring opening polymerization of N ‐carboxyanhydrides. The obtained block ionomers are used to complex pDNA resulting in the formation of polyplexes (PeptoPlexes). The PeptoPlexes can be successfully applied in the transfection of HEK 293 T cells and are able to transfect up to 50% of cells in vitro (FACS assay), while causing no detectable toxicity in an Annexin V assay. These findings are a first indication that PeptoPlexes may be a suitable alternative to PEG based non‐viral transfection systems.