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Chitosan Grafted with Phosphorylcholine and Macrocyclic Polyamine as an Effective Gene Delivery Vector: Preparation, Characterization and In Vitro Transfection
Author(s) -
Li Ling,
Zhao Fangfang,
Zhao Baojing,
Zhang Jin,
Li Chao,
Qiao Renzhong
Publication year - 2015
Publication title -
macromolecular bioscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.924
H-Index - 105
eISSN - 1616-5195
pISSN - 1616-5187
DOI - 10.1002/mabi.201400518
Subject(s) - cyclen , chemistry , chitosan , agarose gel electrophoresis , gene delivery , phosphorylcholine , circular dichroism , transfection , nuclear chemistry , stereochemistry , biochemistry , dna , gene
Herein, an effective gene delivery vector phosphorylcholine and macrocyclic polyamine grafted chitosan (PC‐ g (6)‐Cs‐ g (2)‐Cyclen) was developed. Chemical characterization of product PC‐ g (6)‐Cs‐ g (2)‐Cyclen was performed by NMR, FT‐IR, gel permeation chromatography (GPC), and X‐ray photoelectron spectroscopy (XPS) analysis. PC‐ g (6)‐Cs‐ g (2)‐Cyclen could more efficiently bind and protect plasmid DNA than macrocyclic polyamine grafted chitosan (Cs‐ g ‐Cyclen) and phosphorylcholine grafted chitosan (Cs‐ g ‐PC), as evaluated by agarose gel electrophoresis, circular dichroism spectra, and fluorescence quenching assays. PC‐ g (6)‐Cs‐ g (2)‐Cyclen could wrap DNA into uniform nanoparticles in the size of 112.6 ± 8.5 nm and possessed net cationic charge. UV spectroscopy and MTT assays showed excellent water‐solubility and cell viability for PC‐ g (6)‐Cs‐ g (2)‐Cyclen. In addition, three polymer/DNA complexes showed 5.1−15.1‐fold greater uptake activity and 10−14‐fold higher transfection efficiency in 293 T cells as compared to chitosan/DNA complex, in which PC‐ g (6)‐Cs‐ g (2)‐Cyclen demonstrated the highest transfection activity. These date demonstrated that PC‐ g (6)‐Cs‐ g (2)‐Cyclen is a promising vector candidate for gene delivery.