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Mechanical Responses of Cancer Cells on Nanoscaffolds for Adhesion Size Control
Author(s) -
Park Soyeun,
Bastatas Lyndon,
Matthews James,
Lee Yong Joong
Publication year - 2015
Publication title -
macromolecular bioscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.924
H-Index - 105
eISSN - 1616-5195
pISSN - 1616-5187
DOI - 10.1002/mabi.201400504
Subject(s) - metastasis , cancer cell , prostate cancer , adhesion , atomic force microscopy , focal adhesion , cancer , nanotechnology , cancer metastasis , materials science , biophysics , microbiology and biotechnology , cancer research , chemistry , medicine , signal transduction , biology , composite material
A mechano‐reciprocal interaction plays a critical role for cancer cells searching for favorable metastasis sites. For this study, we utilized nanoscaffolds that can control the maturation of focal adhesions in order to investigate how cancer cells mechanically respond to their nanoenvironments. We found that prostate cancer cells showed linearly decreasing proliferation rate and mechanical stiffness as the size of nanoislands on nanoscaffolds where the cells were grown decreases. This mechanical signature was exacerbated for less metastatic prostate cancer cells. However, there was no dependence of mechanical responses on the geometric properties of nanoscaffolds for breast cancer cells, despite the acute inhibition of adhesion and the abrupt mechanical changes. We believe that our holistic approach that utilizes atomic force microscopy (AFM) and nanoscaffolds can reveal which mechano‐reciprocal interactions are crucial for metastasis and, thus, provide useful information for anti‐cancer drug development targeting integrin‐associated signaling.